Investigation of a side reaction occurring during N-linked glycan labeling by cationic tags
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F18%3A00111750" target="_blank" >RIV/00216224:14310/18:00111750 - isvavai.cz</a>
Alternative codes found
RIV/68081715:_____/18:00493551 RIV/62157124:16370/18:43877045
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0021967318309506" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0021967318309506</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.chroma.2018.07.066" target="_blank" >10.1016/j.chroma.2018.07.066</a>
Alternative languages
Result language
angličtina
Original language name
Investigation of a side reaction occurring during N-linked glycan labeling by cationic tags
Original language description
Capillary electrophoresis-mass spectrometry was applied for the analysis of oligosaccharides and N-linked glycans with an attached charge label facilitating electrophoretic migration and electrospray ionization efficiency. Several different labeling strategies have been tested with different tags and tagging reactions including reductive amination and hydrazone formation. However, a formation of multiple labeled N-linked glycans was observed by CE-MS in a positive ion mode when positively charged labels such as aliphatic amines containing a quaternary ammonium group were attached to N-linked glycans by reductive amination. A reaction mechanism explaining a side reaction occurring during the labeling and the multiple product formation was proposed and confirmed by using isotopically labeled N-acetylglucosamine. Finally, it was confirmed that derivatization of sugars via a hydrazone formation can be a simpler method with a high reaction yield suitable for high sensitive CE-ESI/MS analyses of N-linked glycans.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Chromatography A
ISSN
0021-9673
e-ISSN
1873-3778
Volume of the periodical
1570
Issue of the periodical within the volume
OCT
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
8
Pages from-to
67-74
UT code for WoS article
000445982400008
EID of the result in the Scopus database
2-s2.0-85050934138