Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F18%3A00111972" target="_blank" >RIV/00216224:14310/18:00111972 - isvavai.cz</a>
Alternative codes found
RIV/86652079:_____/18:00493286
Result on the web
<a href="https://www.jove.com/video/58076/spectrophotometric-determination-phycobiliprotein-content" target="_blank" >https://www.jove.com/video/58076/spectrophotometric-determination-phycobiliprotein-content</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3791/58076" target="_blank" >10.3791/58076</a>
Alternative languages
Result language
angličtina
Original language name
Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis
Original language description
This is a simple protocol for the quantitative determination of phycobiliprotein content in the model cyanobacterium Synechocystis. Phycobiliproteins are the most important components of phycobilisomes, the major light-harvesting antennae in cyanobacteria and several algae taxa. The phycobilisomes of Synechocystis contain two phycobiliproteins: phycocyanin and allophycocyanin. This protocol describes a simple, efficient, and reliable method for the quantitative determination of both phycocyanin and allophycocyanin in this model cyanobacterium. We compared several methods of phycobiliprotein extraction and spectrophotometric quantification. The extraction procedure as described in this protocol was also successfully applied to other cyanobacteria strains such as Cyanothece sp., Synechococcuselongatus, Spirulina sp., Arthrospira sp., and Nostoc sp., as well as to red algae Porphyridium cruentum. However, the extinction coefficients of specific phycobiliproteins from various taxa can differ and it is, therefore, recommended to validate the spectrophotometric quantification method for every single strain individually. The protocol requires little time and can be performed in any standard life science laboratory since it requires only standard equipment.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/LM2015055" target="_blank" >LM2015055: Center for Systems Biology</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
ISSN
1940-087X
e-ISSN
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Volume of the periodical
Neuveden
Issue of the periodical within the volume
139
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
1-9
UT code for WoS article
000456210900004
EID of the result in the Scopus database
2-s2.0-85054377142