Capillary electrophoresis, a method for the determination of nucleic acid ligands covalently attached to quantum dots representing a donor of Forster resonance energy transfer

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F18%3A00112184" target="_blank" >RIV/00216224:14310/18:00112184 - isvavai.cz</a>
Alternative codes found
RIV/68081715:_____/18:00489805 RIV/00216208:11310/18:10391610 RIV/26475821:_____/18:N0000004
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/pdf/10.1002/jssc.201800248" target="_blank" >https://onlinelibrary.wiley.com/doi/pdf/10.1002/jssc.201800248</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jssc.201800248" target="_blank" >10.1002/jssc.201800248</a>

Result language
angličtina
Original language name
Capillary electrophoresis, a method for the determination of nucleic acid ligands covalently attached to quantum dots representing a donor of Forster resonance energy transfer
Original language description
The synthesis and determination of the structure of a Forster resonance energy transfer probe intended for the detection of specific nucleic acid sequences are described here. The probe is based on the hybridization of oligonucleotide modified quantum dots with a fluorescently labeled nucleic acid sample resulting in changes of the fluorescence emission due to the energy transfer effect. The stoichiometry distribution of oligonucleotides conjugated to quantum dots was determined by capillary electrophoresis separation. The results indicate that one to four molecules of oligonucleotide are conjugated to the surface of a single nanoparticle. This conclusion is confirmed by the course of the dependence of Forster resonance energy transfer efficiency on the concentration of fluorescently labeled complementary single-stranded nucleic acid, showing saturation. While the energy transfer efficiency of the probe hybridized with complementary nucleic acid strands was 30%, negligible efficiency was observed with a noncomplementary strand.
Czech name
—
Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry

Project
<a href="/en/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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