Tobacco BY-2 cell suspension as a model for assessment of pharmaceuticals phytotoxicity
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F19%3A00107656" target="_blank" >RIV/00216224:14310/19:00107656 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Tobacco BY-2 cell suspension as a model for assessment of pharmaceuticals phytotoxicity
Original language description
Pharmaceuticals and pharmaceutically active substances nowadays represent an important class of emerging environmental pollutants. Growth and developmental changes in plants induced by pharmaceuticals reflect changes of processes at the cellular and subcellular levels. Due to their growth and cellular characteristics, plant cell suspension cultures like Nicotiana tabacum BY-2 can be a suitable model for assessing toxicity. Non-steroidal anti-inflammatory drug diclofenac (DCF) belongs to currently monitored compounds in the environment because of its frequent occurrence and high potential toxicity. This study suggests that exposure to environmentally relevant concentrations of DFC (1, 10, and 100 ug/L) affected significantly the viability of BY-2 cells, suspension density and mitotic index. The growth and viability changes after 120 h of treatment were accompanied by increased production of total reactive oxygen species (ROS) and mitochondrial superoxide, and by down-regulation of reactive nitrogen species and total thiols in correlation to DCF treatment. DCF-induced oxidative stress triggered apoptotic alterations in BY-2 cells leading to programmed cell death – irregular or stretched nuclei were observed in up to 50% of cells. The response of the tobacco BY-2 cell suspension to DCF treatment is prompt and sensitive and selected parameters are suitable for toxicity evaluation.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
<a href="/en/project/GF17-33746L" target="_blank" >GF17-33746L: Drugs in the environment and interaction with plants</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů