Microbial Contamination of Ancient Human Dental Calculus Studied by Scanning Electron Microscopy and Metagenomic Approach
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F19%3A00110713" target="_blank" >RIV/00216224:14310/19:00110713 - isvavai.cz</a>
Result on the web
<a href="http://www.pojivo.cz/pu/PU-2019-Suppl-2--Humpolec-190830.pdf" target="_blank" >http://www.pojivo.cz/pu/PU-2019-Suppl-2--Humpolec-190830.pdf</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Microbial Contamination of Ancient Human Dental Calculus Studied by Scanning Electron Microscopy and Metagenomic Approach
Original language description
Dental calculus has great potential in the research of oral microbiome in past populations. Alongside the advantages of novel molecular approaches applied on ancient human dental calculus, we encounter the drawbacks as well. One of the main issues is contamination. There is a possibility of detection of microbial contamination by scanning electron microscopy prior to subsequent expensive molecular analysis. Although decontamination is always part of the workflow, the metagenomic analysis points out the residual microbial contamination. This study presents the means of detecting contamination and precautions to be taken for the best prevention of it.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů