Quaternary protoberberine alkaloids and their interactions with DNA
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F19%3A00112242" target="_blank" >RIV/00216224:14310/19:00112242 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/article/10.1007%2Fs11696-019-00857-z" target="_blank" >https://link.springer.com/article/10.1007%2Fs11696-019-00857-z</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11696-019-00857-z" target="_blank" >10.1007/s11696-019-00857-z</a>
Alternative languages
Result language
angličtina
Original language name
Quaternary protoberberine alkaloids and their interactions with DNA
Original language description
The G-quadruplex DNA is commonly present in several protooncogenic-DNA promoters and participates in many important biological processes such as replication, transcription, and translation. Because of their supposed role in cancer, G-quadruplex DNA is often studied as a target for anti-cancer drugs. Quaternary protoberberine and tetrahydroprotoberberine alkaloids (corysamine, coptisine, stylopine), which are supposed to selectively bind these structures, have been compared in terms of stability with selected types of DNA. Influence of selected alkaloids on the stability of double-stranded DNA and non-canonical forms of DNA was observed by determining association constants of alkaloid-DNA complexes using spectroscopic methods-molecular absorption spectrometry, fluorescence spectrometry, and mass spectrometry. Furthermore, the effect of given alkaloids on the melting temperature of these DNA structures was determined using CD spectrometry. Competitive dialysis and electrospray mass spectrometry were performed for affinity comparison of certain alkaloids to different DNA structures including G-quadruplexes. These experiments have proven that corysamine and coptisine prefer interaction with G-quadruplexes in comparison to dsDNA and ssDNA, whereas tetrahydroprotoberberine alkaloid stylopine does not interact with any DNA whatsoever.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: Central european institute of technology</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Chemical Papers
ISSN
0366-6352
e-ISSN
1336-9075
Volume of the periodical
73
Issue of the periodical within the volume
12
Country of publishing house
CH - SWITZERLAND
Number of pages
9
Pages from-to
2965-2973
UT code for WoS article
000488930200010
EID of the result in the Scopus database
2-s2.0-85073203733