Histopathology of laboratory-reared Nothobranchius fishes: Mycobacterial infections versus neoplastic lesions
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F21%3A00119124" target="_blank" >RIV/00216224:14310/21:00119124 - isvavai.cz</a>
Alternative codes found
RIV/68081766:_____/21:00541916 RIV/00209805:_____/21:00078674 RIV/00216208:11310/21:10431352
Result on the web
<a href="https://doi.org/10.1111/jfd.13378" target="_blank" >https://doi.org/10.1111/jfd.13378</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/jfd.13378" target="_blank" >10.1111/jfd.13378</a>
Alternative languages
Result language
angličtina
Original language name
Histopathology of laboratory-reared Nothobranchius fishes: Mycobacterial infections versus neoplastic lesions
Original language description
Short-lived killifishes of the genus Nothobranchius Peters, 1868 (Cyprinodontiformes) are considered promising model organisms for biomedical research on ageing and tumorigenesis. We conducted histopathological analysis of 411 adult individuals from three Nothobranchius species to study details on spontaneous age-related neoplastic lesions. Light microscopy based on H&E and toluidine blue-stained sections revealed (a) non-proliferative liver changes with pronounced vacuolation of hepatocytes; (b) proliferation of kidney haemopoietic tissue contributing to excretory system damage; (c) proliferation of splenic mononuclear haemoblasts accompanied by reduced erythropoiesis; (d) proliferation of mononuclear cell aggregates in the liver parenchyma; and (e) rare occurrence of hepatocellular adenomas. Ziehl–Neelsen (ZN) staining revealed that the proliferative lesions are a host defence response to mycobacterial infections manifested by activation of the mononuclear phagocytic system and atypical granulomatous inflammatory reaction. 16S rRNA analysis identified three species of Mycobacterium in our samples. Our findings turn attention to lesions which mimic neoplasms by their gross appearance and question the light microscopic interpretation of lesions unless differential ZN staining is included. Beyond the limitations of our morphological approach, the intensity of mycobacterial infections is a challenging opportunity for research into the molecular-genetic background of the mononuclear phagocytic system reaction in Nothobranchius killifish.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10617 - Marine biology, freshwater biology, limnology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Fish Diseases
ISSN
0140-7775
e-ISSN
—
Volume of the periodical
44
Issue of the periodical within the volume
8
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
1179-1190
UT code for WoS article
000639234500001
EID of the result in the Scopus database
2-s2.0-85104149796