Changes of peptidase profile during Fasciola hepatica embryonation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F21%3A00119646" target="_blank" >RIV/00216224:14310/21:00119646 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Changes of peptidase profile during Fasciola hepatica embryonation
Original language description
Fasciola hepatica is a widespread foodborne trematode that parasitizes a broad range of mammals, including humans. Adults of this cosmopolitan species lay their propagation stages - unsegmented operculated eggs, into the bile duct of their mammalian host. Crucial aspects of F. hepatica life cycle, such as migration, reproduction and immune evasion, are directly dependent on the activity of proteolytic enzymes, also known as peptidases. Expression of these molecules have been intensely studied in metacercariae, newly excysted juveniles and adults, however embryonic stages of F. hepatica, have not yielded much scientific attention in this context. We used combined transcriptomic/proteomic approach which led to the identification of expressed and translated peptidases in developing eggs of different age. Additionally, activity profiling with the substrate library and selective inhibitors identified presence of different peptidase classes in egg extracts. Our results indicate that F. hepatica egg transcriptome remains mostly constant, while the proteome is being considerably adjusted throughout the embryonation. Threonine catalytic subunits of proteasome are constantly produced, while the cysteine peptidases prevailing in freshly laid eggs are supplemented by aspartic peptidases and metallopeptidases in the later stages of egg development.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/GA19-17269S" target="_blank" >GA19-17269S: Proteolysis in eggs of parasitic worms: role in pathology and its regulation</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů