Determination of 8-hydroxy-2 '-deoxyguanosine in urine with "linear" mode sparked graphite screen-printed electrodes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F21%3A00122813" target="_blank" >RIV/00216224:14310/21:00122813 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0013468621016613?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0013468621016613?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.electacta.2021.139371" target="_blank" >10.1016/j.electacta.2021.139371</a>
Alternative languages
Result language
angličtina
Original language name
Determination of 8-hydroxy-2 '-deoxyguanosine in urine with "linear" mode sparked graphite screen-printed electrodes
Original language description
The development of sensors for diagnostic purposes is of immense importance. Despite the number of proposed sensors showing attractive detection capabilities, the widespread use of these sensors is mainly hindered by selectivity issues and the elaborate tailoring of the sensing surface that eventually increases both the cost of the final sensor and the individual analysis cost. Herein, we report on the development of an advanced sensor for 8−hydroxy−2ˊ−deoxyguanosine (8−OHdG), an important biomarker for DNA oxidative damage. The sensor was developed on a low-cost graphite screen-printed electrode (SPE) by employing for the first time, an extremely fast SPE-to-graphite “linear” mode sparking process that enables the uniform modification of the whole electrode surface in less than 20 s. Graphite-sparked SPEs exhibited a linear relationship with the concentration of 8−OHdG over the range 2–50 nM, while the limit of detection (3σ) was 0.35 nM. The sensors showed a notable resistance to interference by 1000-fold excess of dopamine and ascorbic acid, and 100-fold excess of uric acid. A simple treatment of the samples, based on uricase, that eliminates the interference caused by uric acid under real-world conditions (>1000-fold excess) was optimized and proposed. The method was successfully applied to the determination of 8−OHdG in synthetic urine samples. Recovery was 95%.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrochimica Acta
ISSN
0013-4686
e-ISSN
1873-3859
Volume of the periodical
399
Issue of the periodical within the volume
December
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
9
Pages from-to
1-9
UT code for WoS article
000711318400001
EID of the result in the Scopus database
2-s2.0-85117086414