OPTIMIZATION OF NUCLEIC ACID BINDING TO MAGNETIC PARTICLES WITH THE AIM OF DETECTION OF DANGEROUS VIRUSES

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F21%3A00129991" target="_blank" >RIV/00216224:14310/21:00129991 - isvavai.cz</a>
Result on the web
<a href="https://www.confer.cz/nanocon/2020/3748-optimization-of-nucleic-acid-binding-to-magnetic-particles-with-the-aim-of-detection-of-dangerous-viruses" target="_blank" >https://www.confer.cz/nanocon/2020/3748-optimization-of-nucleic-acid-binding-to-magnetic-particles-with-the-aim-of-detection-of-dangerous-viruses</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.37904/nanocon.2020.3748" target="_blank" >10.37904/nanocon.2020.3748</a>

Result language
angličtina
Original language name
OPTIMIZATION OF NUCLEIC ACID BINDING TO MAGNETIC PARTICLES WITH THE AIM OF DETECTION OF DANGEROUS VIRUSES
Original language description
African Swine Fever Virus (ASFV) is a DNA virus of the Asfivirus genus of the Asfarviridae family that is found in blood, body fluids, and internal organs. ASFV was described more than 40 years ago. This virus spreads pandemically and the mortality rate of the virus-related disease ranges from 90 to 100 %. The aim of this study was to propose the detection of specific nucleic acid of ASFV using electrochemical hybridization biosensor. Determination of DNA was conducted by AdTSV DPV a CV (potential 0 V, end potential -1.8 V, step potential 5 mV, modulation amplitude 25 mV, 0.2 M acetate buffer pH 5.0). The volume of analysed sample was 10 mu L. CV signals CA (log -0.0373x, r 0.99, Ep -1.30 V) and P peak (log -0.0801x, r 0.99, Ep -1.52 V) were observed. To increase the sensitivity, a modification of ODN with CdTe was proposed. The CdTe signal was observed around the potential of -0.56 V and for modified ODNs the signal was -0.58 V. SPION were prepared to capture DNA. The interaction of DNA (PCR fragment, 280 bp) with SPION was very fast within 30 s. The technique will be further used for a microfluidic system.
Czech name
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Czech description
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Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
Proceedings 12th International Conference on Nanomaterials - Research & Application
ISBN
9788087294987
ISSN
2694-930X
e-ISSN
—
Number of pages
6
Pages from-to
324-329
Publisher name
TANGER LTD
Place of publication
Ostrava
Event location
Brno
Event date
Oct 21, 2020
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000664505500055

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