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Advanced Microscopic and Biosensing Approaches for Monitoring Bacteriophage-Mediated Lysis of Bacteria

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F23%3A00131316" target="_blank" >RIV/00216224:14310/23:00131316 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Advanced Microscopic and Biosensing Approaches for Monitoring Bacteriophage-Mediated Lysis of Bacteria

  • Original language description

    Phage therapy uses lytic phages to kill the bacterial host as a result of the virus life cycle, thus working with dynamic, living, and evolving entities. For the development and optimization of effective phage-based treatments, it is essential to have a thorough understanding of the mechanisms that participate in the process of phage-mediated bacterial lysis. Promising new approaches such as atomic force microscopy (AFM) and surface plasmon resonance (SPR) biosensing can be utilized for this purpose. Both are sensitive and rapid analytical methods that provide detailed insight into how phages interact with bacterial cells and allow for monitoring immobilized cells under native conditions in real-time. In our study, we investigated the efficacy of lytic antimicrobials against the pathogenic bacterium Staphylococcus aureus. Specifically, we explored the mechanisms of bacterial lysis mediated by the phage vB_SauP_P68 (P68) and the enzyme lysostaphin. AFM enabled a high-resolution investigation of topographical and biomechanical properties at the single-cell level. The SPR experiments completed the work with the information on the kinetics of agent-bacterium interaction. The results are expected to advance the development of this promising field, combat bacterial infections and support the exploitation of phage therapy.

  • Czech name

  • Czech description

Classification

  • Type

    O - Miscellaneous

  • CEP classification

  • OECD FORD branch

    10608 - Biochemistry and molecular biology

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2023

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů