Boxeed: Precision Seeding and seed Phenotyping

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F23%3A00131422" target="_blank" >RIV/00216224:14310/23:00131422 - isvavai.cz</a>
Result on the web
<a href="https://www.acpd2023.org/" target="_blank" >https://www.acpd2023.org/</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Boxeed: Precision Seeding and seed Phenotyping
Original language description
Many Arabidopsis research programs are focused on the detailed characterization of early developmental growth, followed by costly and laborious omics data processing. To make the most of such datasets, it is crucial to reduce any source of variability. One of the most challenging sources of variability is the micro-sized seeds and their manual handling. Here, we introduce Boxeed, a state-of-the-art technology intended for non-invasive, dry-seed processing including phenotyping, sorting, counting as well as their precise seeding to growth media. Boxeed is designed for seeds between 80 µm – 3 mm, reaching average working speeds of 600 seeds/hour. Phenotyping is based on 2D image analysis of individual seeds from multiple projections. Seed morphometric and fluorescence parameters are calculated in real time, making it ideal for seed selection and seed sorting directly from stocks. Using seed phenotyping and precise seed-to-seed positioning in Arabidopsis thaliana, we have identified significant variations in early postembryonic growth, which arise due to differences in seed size and seed-to-seed distance. Boxeed's seed sorting and precise seeding capabilities can reduce experimental variability thus enabling experimenters correlate hitherto hidden gene functions with phenotypes.
Czech name
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Czech description
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Project
<a href="/en/project/EF16_026%2F0008446" target="_blank" >EF16_026/0008446: Signal integration and epigenetic reprograming for plant productivity</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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