All

What are you looking for?

All
Projects
Results
Organizations

Quick search

  • Projects supported by TA ČR
  • Excellent projects
  • Projects with the highest public support
  • Current projects

Smart search

  • That is how I find a specific +word
  • That is how I leave the -word out of the results
  • “That is how I can find the whole phrase”

Exploring the Icy Treasures: Biosynthetic potential of Antarctic bacteria from James Ross Island.

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F23%3A00132600" target="_blank" >RIV/00216224:14310/23:00132600 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Exploring the Icy Treasures: Biosynthetic potential of Antarctic bacteria from James Ross Island.

  • Original language description

    Background: The growing problem of antimicrobial resistance motivated scientists to re-explore natural sources of bioactive secondary metabolites. Antarctica represents an extreme environment colonized by bacteria with unique adaptation mechanisms allowing them to thrive under harsh conditions. Such adaptations include production of secondary metabolites to inhibit competitors or sustain abiotic stresses, which predestines these microbes as source of natural products for biomedical use. Objectives: The aim of this work was to recover novel bacterial taxa from Antarctic soils to access the biosynthetic potential hidden in yet uncultivated bacteria. The main objective is the activation of silent biosynthetic gene clusters enabling discovery of novel secondary metabolites, mainly through co-cultivation strategies. Methods: Three isolation methods (pre-selection of spore-forming bacteria, low-nutrient and soil-extract based media) were applied to recover novel bacteria from Antarctic soils, predominantly targeting phyla with high biosynthetic potential such as Actinobacteriota, Proteobacteria and Firmicutes. Activation of silent biosynthetic gene clusters was attempted through targeted cultivation and co-cultivation. Screening for bioactive molecules and evaluation of their novelty was achieved by application of genomics, metabolomics, and bioactivity testing. Results: A collection of 917 isolates was established. Recovered isolates were associated with four bacterial phyla including 77 isolates of novel species. Proteobacteria and Actinobacteriota represented the most abundant phyla. Specific media stimulated biosynthesis of several unknown natural products. Eight strains produced antimicrobial compounds against resistant and multidrug-resistant bacterial and fungal pathogens. Importantly, metabolomic profiling indicated that these strains produced several new secondary metabolites, which may be responsible for the observed antimicrobial activities.

  • Czech name

  • Czech description

Classification

  • Type

    O - Miscellaneous

  • CEP classification

  • OECD FORD branch

    10606 - Microbiology

Result continuities

  • Project

    <a href="/en/project/LM2015078" target="_blank" >LM2015078: Czech Polar Research Infrastructure</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2023

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů