PHAGE-ANTIBIOTIC SYNERGY (PAS) EFFECT EVALUATION AND NEW METHODS FOR ITS DETECTION
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F23%3A00132734" target="_blank" >RIV/00216224:14310/23:00132734 - isvavai.cz</a>
Result on the web
<a href="http://www.ccsss.cz/index.php/ccsss/issue/view/41" target="_blank" >http://www.ccsss.cz/index.php/ccsss/issue/view/41</a>
DOI - Digital Object Identifier
—
Alternative languages
Result language
angličtina
Original language name
PHAGE-ANTIBIOTIC SYNERGY (PAS) EFFECT EVALUATION AND NEW METHODS FOR ITS DETECTION
Original language description
Current trends in the rise of multidrug-resistant bacteria put pressure on the standardization of alternative methods for treatments of such complicated infections. One of the promising possibilities is phage therapy and its new direction, which is based on a combination of phages with antibiotics. This strategy builds on the synergy effect between these compounds (phage-antibiotic synergy; PAS) [1]. We focused on staphylococcal phage S10 with podoviral morphology and antibiotics affecting cell wall development (Amoxicillin) and protein synthesis (Gentamicin). Their combined effect was assessed by plaque assay and spectrophotometry in 96-well plate format or higher volumes in personal bioreactors. Obtained results were evaluated by recently published methods, i.e., Virulence Index, and Phage Score. In both environments tested (solid and liquid) were observed a synergy effect of amoxicillin and phage S10. The overall phage virulence in 24 hrs intervals increased with the concentration below the minimum inhibitory concentration. The application of gentamicin resulted in a decrement in phage titer, and the ability of phage propagation was lost at higher concentrations. This study brings new insights into the interaction of phages with antibiotics, which is crucial for their combined application as a treatment in the future.
Czech name
—
Czech description
—
Classification
Type
O - Miscellaneous
CEP classification
—
OECD FORD branch
10606 - Microbiology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů