Molecular and serological detection of Borrelia burgdorferi sensu lato in small free-living mammals

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F24%3A00138668" target="_blank" >RIV/00216224:14310/24:00138668 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

čeština

Original language name

Molecular and serological detection of Borrelia burgdorferi sensu lato in small free-living mammals

Original language description

Free living mammals serve as reservoir animals for the spirochetes Borrelia burgdorferi s.l. from which the infection can be transmitted through blood sucking insects to other hosts, in which a disease called Lyme disease can occur. The presence of B. burgdorferi s.l. was determined in 170 small mammals caught in the wild according to the approved experimental project. During the autopsy, tissue samples (heart rinses and kidneys) were taken for antibody detection by the ELISA method and for direct detection of B. burgdorferi s.l. by PCR. The species of the animal was determined by using a morphological key, if there was doubt about the distinction between Apodemus flavicollis and Apodemus sylvaticus, AP PCR was used. The prevalence of antibodies against B. burgdorferi s.l. was 10.5% (18/170) for IgM and 10% (17/170) for IgG without statistically significant difference (p&gt;0.05) between the species, age, sex of animals and year season. The presence of B. burgdorferi s.l. was detected by PCR in 14.7% (25/170) of animals. No statistical significance was found in species, age and sex. The positivity statistically significantly differed in the seasons (p=0.021) with the highest positivity in sp ring and lowest positivity in autumn. We expected both antibody and Bbsl prevalence results around the landfill to be higher than in other areas due to the high presence of rodents in a small area, but the results are consistent with findings from other areas, so landfills are not more dangerous and there is no higher risk of Bbsl spreading. than in, for example, a forest park.

Czech name

Molecular and serological detection of Borrelia burgdorferi sensu lato in small free-living mammals

Czech description

Free living mammals serve as reservoir animals for the spirochetes Borrelia burgdorferi s.l. from which the infection can be transmitted through blood sucking insects to other hosts, in which a disease called Lyme disease can occur. The presence of B. burgdorferi s.l. was determined in 170 small mammals caught in the wild according to the approved experimental project. During the autopsy, tissue samples (heart rinses and kidneys) were taken for antibody detection by the ELISA method and for direct detection of B. burgdorferi s.l. by PCR. The species of the animal was determined by using a morphological key, if there was doubt about the distinction between Apodemus flavicollis and Apodemus sylvaticus, AP PCR was used. The prevalence of antibodies against B. burgdorferi s.l. was 10.5% (18/170) for IgM and 10% (17/170) for IgG without statistically significant difference (p&gt;0.05) between the species, age, sex of animals and year season. The presence of B. burgdorferi s.l. was detected by PCR in 14.7% (25/170) of animals. No statistical significance was found in species, age and sex. The positivity statistically significantly differed in the seasons (p=0.021) with the highest positivity in sp ring and lowest positivity in autumn. We expected both antibody and Bbsl prevalence results around the landfill to be higher than in other areas due to the high presence of rodents in a small area, but the results are consistent with findings from other areas, so landfills are not more dangerous and there is no higher risk of Bbsl spreading. than in, for example, a forest park.

Type

D - Article in proceedings

CEP classification

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OECD FORD branch

30303 - Infectious Diseases

Project

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Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

XXVI. KONFERENCE MLADÝCH VĚDECKÝCH PRACOVNÍKŮ S MEZINÁRODNÍ ÚČASTÍ

ISBN

9788073059576

ISSN

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e-ISSN

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Number of pages

3

Pages from-to

85-87

Publisher name

VETERINÁRNÍ UNIVERZITA BRNO

Place of publication

Brno

Event location

Brno

Event date

Jan 1, 2024

Type of event by nationality

CST - Celostátní akce

UT code for WoS article

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