Diplostomum pseudospathaceum (Trematoda) inducing differential immune geneexpression in asexual and sexual gibel carp (Carassius gibelio)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F24%3A00139754" target="_blank" >RIV/00216224:14310/24:00139754 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Diplostomum pseudospathaceum (Trematoda) inducing differential immune geneexpression in asexual and sexual gibel carp (Carassius gibelio)

Original language description

Parasite-mediated selection is considered one of the potential mechanisms contributing to the coexistence of asexual-sexual complexes. Gibel carp (Carassius gibelio), an invasive fish species in Europe, currently often forms populations composed of asexual (reproducing by gynogenesis) and sexual specimens. We performed the experimental infection of eye-fluke Diplostomum pseudospathaceum (Trematoda) in gynogenetic and sexual gibel carp. The transcriptome profile of the fish spleen was analyzed to reveal the differentially expressed immunity-associated genes (DEGs) related to trematode infection focusing on differences in DEGs between gynogenetic and sexual gibel carp. Gynogenetic fish were more parasitized then sexuals. Although metacercariae of D. pseudospathaceum are situated in an immune-privileged organ, our results showed that eye trematodes may induce a host immune response. DEGs induced by eye-fluke infection were revealed, with various impacts on gynogenetic and sexual gibel carp. Specifically, the majority of DEGs were upregulated in sexual gibel carp, whilst downregulated in gynogenetic gibel carp. Differences in gene regulation between gynogenetic and sexual fish were evidenced in many immunity-associated genes. The following GO terms associated with immunity were recognized for DEGs: immune function, Notch signaling pathway, MAP kinase tyrosine/threonine/phosphatase activity, and chemokine receptor activity. KEGG analyses revealed the importance of the genes involved in 12 immunity-associated pathways: FoxO signaling, adipocytokine signaling, TGF-beta signaling, apoptosis, Notch signaling, C-type lectin receptor signaling, efferocytosis, intestinal immune network for IgA production, insulin signaling, virion – human immunodeficiency virus, Toll-like receptor signaling, and phosphatidylinositol signaling system. Our study indicates the limited potential of asexual fish to cope with higher parasite infection (likely a loss of capacity to induce an effective immune response) and highlights the role of molecular mechanisms associated with immunity for the coexistence of asexual and sexual fish.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

10613 - Zoology

Project

<a href="/en/project/GA22-27023S" target="_blank" >GA22-27023S: Evolutionary and ecological mechanisms of the coexistence of asexual and sexual reproduction in fish</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů