Sampling frequency matters: mapping of the healthy infants' gut microbiome during the first year of life
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F25%3A00142052" target="_blank" >RIV/00216224:14310/25:00142052 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1016/j.crmicr.2025.100470" target="_blank" >https://doi.org/10.1016/j.crmicr.2025.100470</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.crmicr.2025.100470" target="_blank" >10.1016/j.crmicr.2025.100470</a>
Alternative languages
Result language
angličtina
Original language name
Sampling frequency matters: mapping of the healthy infants' gut microbiome during the first year of life
Original language description
Early life events significantly influence the developing gut microbiome, yet the response time and duration of microbiome changes to specific factors, such as vaccination or solid food introduction, remain unclear. Consequently, determining the optimal sampling frequency to monitor gut microbiome development is challenging. This study monitored gut microbiome plasticity using 16S rRNA gene sequencing almost daily in one infant (A) and weekly in 12 others (B–M) during their first year. Changes were linked to external factors and their duration analyzed. Three bacterial colonization groups emerged: "Early-life colonizers," "Re-appearing colonizers," and "Later-colonizers." Weekly sampling underestimated microbiome variability, as individual samples within the same week differed by over 1 Shannon index, and most of the weekly coefficients of variation of different alpha diversity indices in the first 23 weeks were higher than 10 %. Alpha diversity variability decreased with age, but beta diversity variability remained high. Key events like solid food introduction and probiotics caused gradual but significant bacterial composition changes, with effects varying among infants. Sparse weekly sampling hindered a detailed understanding of the impact of maternal microbiome, diet, probiotics, vaccinations, and unforeseen variables. Analysis of weekly variability in alpha and beta diversity suggests that such rare sampling may not be sufficient in terms of the outcomes of interest.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Current Research in Microbial Sciences
ISSN
2666-5174
e-ISSN
2666-5174
Volume of the periodical
9
Issue of the periodical within the volume
January
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
15
Pages from-to
100470
UT code for WoS article
001582768200001
EID of the result in the Scopus database
2-s2.0-105015814499