Quantification of soil microbial functional genes as potential new method in environmental risk assessment of pesticides

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F25%3A00142060" target="_blank" >RIV/00216224:14310/25:00142060 - isvavai.cz</a>

Result on the web

<a href="https://link.springer.com/article/10.1007/s10646-025-02920-w" target="_blank" >https://link.springer.com/article/10.1007/s10646-025-02920-w</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10646-025-02920-w" target="_blank" >10.1007/s10646-025-02920-w</a>

Result language

angličtina

Original language name

Quantification of soil microbial functional genes as potential new method in environmental risk assessment of pesticides

Original language description

Pesticides can have adverse effects on soil microorganisms, but they are underrepresented in the currently required OECD 216 test for environmental risk assessment of plant protection products (PPP). The guideline monitors soil microbial nitrogen transformation over 28 days, potentially missing long-term effects of persistent pesticides. Additionally, nitrate alone may be not sensitive enough to detect disruptions in microbial functions. We investigated whether functional gene analysis could provide a more sensitive bioindicator of pesticide impact. To compare this method with the standard test, we conducted a microcosm experiment following the OECD 216 experimental setup. To capture long-term effects beyond the typical test period, we extended the incubation duration to 56 days. Four different concentrations of the persistent fungicide boscalid were added based on predicted environmental concentration. We also assessed microbial responses to fungicide exposure by measuring classical soil microbial parameters. According to the standard test, boscalid had no harmful long-term effects on soil microbiota. In contrast, our analysis of functional genes found an overall reduction in the acid phosphatase-encoding phoN gene abundance on Day 56, and correspondingly, in acid phosphatase activity in the highest fungicide treatment. Simultaneously, we observed a tendency towards lower fungal abundance based on measured copy numbers of an ITS region of nuclear ribosomal DNA (rDNA) and increased cumulative CO2 production. These results indicate a fungicide-related response of the microbial community and impaired microbial phosphorus cycling. Extending the experimental period to 56 days revealed long-term effects that would have otherwise been undetected under the typical 28-day test duration.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10618 - Ecology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

ECOTOXICOLOGY

ISSN

0963-9292

e-ISSN

1573-3017

Volume of the periodical

34

Issue of the periodical within the volume

7

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

13

Pages from-to

1312-1324

UT code for WoS article

001518276900001

EID of the result in the Scopus database

2-s2.0-105009136110