A combined morphological and genetic survey of helminths in the European green toad Bufotes viridis (Laurenti, 1768) from eastern Slovakia
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F25%3A00143776" target="_blank" >RIV/00216224:14310/25:00143776 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1017/S0031182025100966" target="_blank" >https://doi.org/10.1017/S0031182025100966</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1017/S0031182025100966" target="_blank" >10.1017/S0031182025100966</a>
Alternative languages
Result language
angličtina
Original language name
A combined morphological and genetic survey of helminths in the European green toad Bufotes viridis (Laurenti, 1768) from eastern Slovakia
Original language description
Accurate characterization of helminth communities in amphibian hosts is essential for understanding host-parasite dynamics in changing environments. This study presents an integrative parasitological survey of Bufotes viridis populations in eastern Slovakia, using both morphological and molecular methods. A total of 61 road-killed individuals collected across 13 localities were examined for helminth presence. Only nematodes were detected, encompassing 3 families, Rhabdiasidae, Molineidae and Cosmocercidae. Four nematode species were recorded, Rhabdias rubrovenosa, Oswaldocruzia filiformis, O. ukrainae and Aplectana linstowi, and a further unidentified Cosmocerca species. Notably, R. rubrovenosa seems to be a new or previously misidentified helminth species found in Slovakia. Cosmocercidae represented the most abundant family, while Molineidae occurred scarcely. Each species was characterized genetically - for the members of Rhabdiasidae and Cosmocercidae, partial 18S rDNA, complete ITS1, complete 5.8S rRNA, complete ITS2 and partial 28S rDNA sequences were amplified, whereas for representatives of Molineidae, partial COI sequences were obtained. These results underscore the utility of combining molecular and morphological tools in helminth biodiversity studies and provide updated baseline data on nematode infections in B. viridis within an anthropogenically influenced landscape. Despite visual patterns indicating differences in the community compositions of nematode families between urban and rural localities, multivariate analyses testing revealed no significant differences.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10613 - Zoology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
PARASITOLOGY
ISSN
0031-1820
e-ISSN
1469-8161
Volume of the periodical
152
Issue of the periodical within the volume
13
Country of publishing house
GB - UNITED KINGDOM
Number of pages
17
Pages from-to
1338-1354
UT code for WoS article
001599258000001
EID of the result in the Scopus database
2-s2.0-105018626311