Effect of stationary phase surface chemistry and particle architecture in proteomics

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F25%3A00144324" target="_blank" >RIV/00216224:14310/25:00144324 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1016/j.chroma.2025.465976" target="_blank" >https://doi.org/10.1016/j.chroma.2025.465976</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2025.465976" target="_blank" >10.1016/j.chroma.2025.465976</a>

Result language

angličtina

Original language name

Effect of stationary phase surface chemistry and particle architecture in proteomics

Original language description

The kinetic properties of four columns packed with fully porous particles and three with superficially porous particles were characterized for possible application in proteomic bottom-up analyses. All columns provided an attachment of hydrophobic C18 chains at the surface of the stationary phase. However, they differed in the additional attachment of polar groups and/or endcapping procedure. We have used the retention modeling protocol to explore the separation efficiency and maximal achievable peak capacity on tested columns. Almost all columns provided comparable maximal peak capacity in the range of 500 - 700 for the eight-hour gradient run. This confirms that the family of the stationary phases used in the bottom-up proteomics can be extended. In the case of fully porous particles, we found that the higher the column peak capacity, the higher the number of identified peptides in the simple proteomic sample, with approximately one identified peptide per peak capacity unit. On the contrary, in the case of the superficially porous particles, the number of identified peptides in the sample decreased with the higher column peak capacity. This trend can be overturned only when the lower amount of the sample is injected. Hence, when bottom-up proteomics is considered, the lower loadability of the superficially porous particles still needs to be addressed. Most stationary phases tested can be successfully used in the bottom-up analyses. However, the stationary phases with incorporated polar functional groups reduced the undesirable contribution of free silanol groups to peptide peak tailing and increased the information provided by LC-MS analysis.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10406 - Analytical chemistry

Project

<a href="/en/project/GA23-07581S" target="_blank" >GA23-07581S: Comprehensive top-down-bottom-up proteomics</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Chromatography

ISSN

0021-9673

e-ISSN

1873-3778

Volume of the periodical

1752

Issue of the periodical within the volume

July

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

8

Pages from-to

465976

UT code for WoS article

001484552900001

EID of the result in the Scopus database

2-s2.0-105003582156