Construction of a syntrophic Pseudomonas putida consortium with reciprocal substrate processing

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F25%3A00144580" target="_blank" >RIV/00216224:14310/25:00144580 - isvavai.cz</a>

Result on the web

<a href="https://academic.oup.com/synbio/article/10/1/ysaf012/8172468" target="_blank" >https://academic.oup.com/synbio/article/10/1/ysaf012/8172468</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/synbio/ysaf012" target="_blank" >10.1093/synbio/ysaf012</a>

Result language

angličtina

Original language name

Construction of a syntrophic Pseudomonas putida consortium with reciprocal substrate processing

Original language description

Synthetic microbial consortia can leverage their expanded enzymatic reach to tackle biotechnological challenges too complex for single strains, such as biosynthesis of complex secondary metabolites or waste plant biomass degradation and valorisation. The benefit of metabolic cooperation comes with a catch-installing stable interactions between consortium members. Here, we established a mutualistic relationship in the synthetic consortium of Pseudomonas putida strains through reciprocal processing of two disaccharides-cellobiose and xylobiose-obtainable from lignocellulosic residues. Two strains were engineered to hydrolyse and metabolize these sugars: one grows on xylose and hydrolyses cellobiose to produce glucose, while the other grows on glucose and cleaves xylobiose to produce xylose. This specialization allows each strain to provide essential growth substrate to its partner, establishing a mutualistic interaction, which can be termed reciprocal substrate processing. Key enzymes from Escherichia coli (xylose isomerase pathway) and Thermobifida fusca (glycoside hydrolases) were introduced into P. putida to broaden its carbohydrate utilization capabilities and arranged in a way to instal the strain cross-dependency. A mathematical model of the consortium assisted in predicting the effects of substrate composition, strain ratios, and protein expression levels on population dynamics. Our results demonstrate that modulating extrinsic factors such as substrate concentration can help in balancing fitness disparities between the strains, but achieving this by altering intrinsic factors such as glycoside hydrolase expression levels is much more challenging. This study presents reciprocal substrate processing as a strategy for establishing an obligate dependency between strains in the engineered consortium and offers valuable insights into overcoming the challenges of fostering synthetic microbial cooperation.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

20801 - Environmental biotechnology

Project

<a href="/en/project/GA22-12505S" target="_blank" >GA22-12505S: Synthetic consortium of Pseudomonas putida strains for biodegradation and co-utilisation of (hemi)cellulosic polymers.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Synthetic Biology

ISSN

2397-7000

e-ISSN

2397-7000

Volume of the periodical

10

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

15

Pages from-to

1-15

UT code for WoS article

001547592400001

EID of the result in the Scopus database

2-s2.0-105013312794