Specific Determination of Glutathione using Micellar Elektrokinetic Chromatography and On-Column Reaction with 2,2-dipyridyldisulphide
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F99%3A00003210" target="_blank" >RIV/00216224:14310/99:00003210 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Specific Determination of Glutathione using Micellar Elektrokinetic Chromatography and On-Column Reaction with 2,2-dipyridyldisulphide
Original language description
The on-column reaction of glutathione with the reactive disulphide 2,2-dipyridyldisulphide was used for the specific detection of glutathione during micellar electrokinetic chromatography: In this set-up the front end of the capillary is used as the reaction chamber. 2,2-dipyridyldisulphide and a sample of glutathione are injected consecutively in the capillary as two discrete plugs separated with short plug of background electrolyte. Due to the differences in the mobilities of the 2,2-dipyridyldisulphide and glutathione on column mixing and reaction occur. This reaction is rapid and quantitative, because thiol form is stabilised by thiol-thione tautomerism. This fact also causes a large shift of the absorption maxima toward longer wavelength. Glutathione is thus quantitatively transformed into a mixed disulphide concomitantly with formation of equimolar amounts of the 2-thiopyridone that is further separated by micellar electrokinetic chromatography and determined spectrophotometrical
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA525%2F97%2F0340" target="_blank" >GA525/97/0340: Activity of Thiobacillus ferrooxidans in relation to ecotoxicology of sulfide wastes</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
1999
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Book of Abstract, 1st International Symposium on Separations in the Biosciences, Amsterdam, Netherlands
ISBN
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ISSN
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e-ISSN
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Number of pages
1
Pages from-to
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Publisher name
Elsevier, Netherlands
Place of publication
Amsterdam, Netherlands
Event location
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Event date
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Type of event by nationality
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UT code for WoS article
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