Regulated Expression of Isopentenyl Transferase in Arabidopsis: System Development.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F01%3A00004568" target="_blank" >RIV/00216224:14330/01:00004568 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—
Alternative languages
Result language
angličtina
Original language name
Regulated Expression of Isopentenyl Transferase in Arabidopsis: System Development.
Original language description
A novel system allowing a non-invasive manipulation of cytokinin levels is being developed in A. thaliana. Transcriptional fusions between isopentenyl transferase gene, ipt, and a reporter gene, GUS, were inserted behind a synthetic promoter (pOp). pOp is expected to be inactive when introduced into wild type plants but is activated when these plants are crossed with others that express a novel transcription activation protein, LhG4. Transgenic Arabidopsis plants carrying pOp-ipt-GUS and pOp-GUS-ipt were generated by Agrobacterium infiltration technique. The transformed plants developed without any detectable phenotype alteration indicating that pOp promoter is virtually silent in the transformants in the absence of LhG4. The first evidence for activation of ipt and GUS in pOp-ipt-GUS and pOp-GUS-ipt was provided by crossing the individual transformants with an activator line carrying LhG4 under control of CaMV35S promoter (CaMV35S-LhG4). We will report phenotype alterations observed i
Czech name
—
Czech description
—
Classification
Type
D - Article in proceedings
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
—
Result continuities
Project
<a href="/en/project/IAA5004001" target="_blank" >IAA5004001: Transkription activation system for studying the relationship between cytokinin metabolism and action in Arabidopsis and tobacco</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2001
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
17th International Conference on Plant Growth Substances, Brno, Czech Republic, July 1-6, 2001, Abstracts
ISBN
80-7157-518-6
ISSN
—
e-ISSN
—
Number of pages
1
Pages from-to
88
Publisher name
Mendel University of Agriculture and Forestry, Brno
Place of publication
Brno
Event location
Brno
Event date
Jan 1, 2001
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
—