Colocalization of PML bodies and PML/RARalpha microspeckles with up- and down-regulated loci and changes of chromatin structure in APL leukemia cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F04%3A00009698" target="_blank" >RIV/00216224:14330/04:00009698 - isvavai.cz</a>

Alternative codes found

RIV/68081707:_____/04:00108438

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Colocalization of PML bodies and PML/RARalpha microspeckles with up- and down-regulated loci and changes of chromatin structure in APL leukemia cells

Original language description

The reciprocal translocation between PML and RARalpha genes associated with acute promyelocytic leukemia (APL) results in disintegration of PML bodies and expression of the fusion protein PML/RARalpha in transformed APL cells. Transcription intensity ofparticular genes were compared between Zn-treated (APL) and untreated (healthy) U937 PIR9 cells, stably transfected by the expression vector carrying the PML/RARalpha fusion gene under the control of Zn-inducible promoter; downregulation of many genes clustered in the locus 19p13.3 and upregulation of some of those in Xq28-Xqter were revealed by microchip analysis and RT-PCR after zinc treatment. We have examined the colocalization of these up- (Xq28-qter)-regulated (19p13.3)chromosomal loci with PML bodies in normal and PML/RARalpha microspeckles in transformed APL cells respectively, as well as the changes in chromatin structure of these loci induced in APL cells by PML/RARalpha-altered transcription. Most frequently, colocalization o

Czech name

Kolokalizace PML tělísek a PML/RARalpha mikroshluků s up- a down- regulovanými lokusy a změny struktury chromatinu u APL leukemických buněk

Czech description

Kolokalizace PML tělísek a PML/RARalpha mikroshluků s up- a down- regulovanými lokusy a změny struktury chromatinu u APL leukemických buněk.

Type

D - Article in proceedings

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Biophysics of the Genome

ISBN

80-210-3560-9

ISSN

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e-ISSN

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Number of pages

16

Pages from-to

8-23

Publisher name

Masaryk University

Place of publication

Brno

Event location

Brno

Event date

Oct 12, 2004

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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