Detection of chromosome 13 abnormalities and 14q32 translocations in multiple myeloma using simultaneous imunofluorescent labelling of malignant plasma cells and fluorescent in situ hybridization
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F04%3A00018997" target="_blank" >RIV/00216224:14330/04:00018997 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Detection of chromosome 13 abnormalities and 14q32 translocations in multiple myeloma using simultaneous imunofluorescent labelling of malignant plasma cells and fluorescent in situ hybridization
Original language description
Chromosomal aberrations such as 13q14 deletions or translocations involving 14q32 are described to be common cytogenetic findings in multiple myeloma (MM). Especially, deletions of 13q14 has been associated with an adverse outcome and it has been proposed as one of the most important prognostic factors for MM patients. Because metaphase cytogenetic studies in MM are hampered by a low proliferative activity of myeloma cells in vitro, interphase fluorescent in situ hybridization (FISH) using specific DNAprobes is the technique most widely used for the determination of genomic aberrations in this disease. In the present study we have performed fluorescence in situ hybridization experiments with probes directed to the 13q14 and 14q32 chromosomal regions in 30 patients with MM. For identification of malignant plasma cells in bone marrow samples, we have used cytoplasmic immunoglobulin (cIg) labelling methodology (Ahmann et al. 1998) This method allowed us to identify simultaneously monotyp
Czech name
Studium delece/monozomie chromozomu 13 a translokace 14q32 u pacientů s mnohočetným myelomem pomoci imunofluorescenčního značení maligních plazmatických buněk a techniky FISH.
Czech description
Práce se zabývá detekcí prognosticky významných chromozomových abnormalit u pacient s mnohoetným myelomem (delece/monozomie chromozomu 13, translokace 14q32) pomocí imunofluorescenního znaení maligních plazmatických bunk v kostní deni a techniky FISH.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/NR8183" target="_blank" >NR8183: Molecular-cytogenetic analysis of marked plasma cells and prognostic significance of clonal chromosomal aberrations in multiple myeloma</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2004
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
European Journal of Human Genetics
ISSN
1018-4813
e-ISSN
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Volume of the periodical
12
Issue of the periodical within the volume
Suppl. 1
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
1
Pages from-to
170
UT code for WoS article
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EID of the result in the Scopus database
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