Obtaining DNA markers for diversity evaluation in Trifolium pratense cultivars

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F05%3A00012699" target="_blank" >RIV/00216224:14330/05:00012699 - isvavai.cz</a>

Alternative codes found

RIV/26296080:_____/05:00000305

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Obtaining DNA markers for diversity evaluation in Trifolium pratense cultivars

Original language description

Red clover (Trifolium pratense L.) is one of the main forage species of temperate regions. Cultivars of red clover are allogamous and heterogeneous which their genetic analysis makes difficult. The objective of this study was obtaining DNA markers (RAPD,SSR) for genetic diversity evaluation among red clover cultivars and verification of utility of SSR oligonucleotides from white clover (T. repens). RAPD (Random Amplified Polymorphic DNA) requires no knowledge of DNA sequences for primers designing. SSRs (Simple Sequence Repeats) are highly informative codominant molecular markers which are well known in white clover. Red clover cv. Kvarta and Start and white clover cv. Hájek and Jura were used for molecular analysis. Genomic DNA of red and white clover plants was extracted by bulking method from different plant numbers. CTAB-based method and GenEluteTMPlant Genomic DNA Kit (Sigma) were used for DNA isolation. RAPD analysis was performed with 20 primers and four PCR programmes from lit

Czech name

Získání DNA markerů pro hodnocení diverzity odrůd Trifolium pratense

Czech description

Jetel luční (Trifolium pratense L.) je jednou z hlavních pícnin. Jde o druh cizosprašný, u něhož jednotlivé odrůdy jsou po genetické a molekulární stránce heterogenní a jejich analýza je obtížná. Cílem práce bylo získání DNA markerů (RAPD, SSR) pro hodnocení genetické diverzity odrůd.

Type

D - Article in proceedings

CEP classification

GE - Plant cultivation

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Book of Abstracts, 6th International Symposium in the Series Recent Advances in Plant Biotechnology, 12.-16.9.2005

ISBN

80-86778-16-9

ISSN

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e-ISSN

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Number of pages

1

Pages from-to

42

Publisher name

Institute of Plant Molecular Biology ASCR

Place of publication

České Budějovice

Event location

České Budějovice

Event date

Jan 1, 2005

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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