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Technique of light chain-specific immunofluorescent staining of clonal plasma cells and FISH analysis

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F06%3A00018067" target="_blank" >RIV/00216224:14330/06:00018067 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Technique of light chain-specific immunofluorescent staining of clonal plasma cells and FISH analysis

  • Original language description

    MULTIPLE MYELOMA (MM) is characterized by monoclonal proliferation of plasma cells and represents approximately 10% of all hematologic malignancies. The clinical course of patients with MM is highly variable. Reliable genetic indicators are therefore required for their stratification and for more adequate therapy. The metaphase cytogenetic studies in MM are hampered by a low proliferative activity of myeloma cells in vitro. Therefore, interphase fluorescent in situ hybridization (I-FISH) using specificDNA probes is the technique of choice for the determination of genomic aberrations associated with this disease. However, I-FISH on bone marrow specimens do not distinguish malignant plasma cells. In order to solve this problem, various novel techniqueshave been designated for selection of myeloma cells. Immunofluorescent labelling of malignant plasma cells is suitable approach for the diagnosis of chromosomal abnormalities in patients with MM. This method allows selection of monotypic

  • Czech name

    Technika imunofluoresenčního znační klonálních plazmatických buněk u mnohočetného myelomu a FISH

  • Czech description

    Práce se zabývá identifikací plazmatických buněk u mnohočetného myelomu pomocí imunofluorescenčního barvení cytoplazmatických imunoglobulínů v kombinaci s technikou FISH.

Classification

  • Type

    D - Article in proceedings

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/NR8183" target="_blank" >NR8183: Molecular-cytogenetic analysis of marked plasma cells and prognostic significance of clonal chromosomal aberrations in multiple myeloma</a><br>

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2006

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    Detection of chromosomal aberrations in multiple myeloma

  • ISBN

    80-210-4129-3

  • ISSN

  • e-ISSN

  • Number of pages

    1

  • Pages from-to

    16

  • Publisher name

    Masaryk University

  • Place of publication

    Brno

  • Event location

    Brno, ILBIT MU, Kamenice 5

  • Event date

    Oct 25, 2006

  • Type of event by nationality

    EUR - Evropská akce

  • UT code for WoS article