Optimizing light throughput in fluorescence microscopy
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F06%3A00041813" target="_blank" >RIV/00216224:14330/06:00041813 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—
Alternative languages
Result language
angličtina
Original language name
Optimizing light throughput in fluorescence microscopy
Original language description
Fluorescence microscopy applications often suffer from the lack of light. Low light levels typically cause poor signal-to-noise ratio or long exposure times. Signal level in fluorescence set-up is determined by many factors, such as: power of the light source and its spectral characteristics, transmission curves of excitation/emission filters, transmission curves of and dichroic/polychroic mirrors, excitation/emission spectra of imaged fluorochrome(s), numerical aperture of the objective and its transmission curve, quantum efficiency curve of the light detector. Thus, resulting light throughput is given by the composition of spectral characteristics (functions of wavelength) of many components. Proper computation and optimization of light throughput isa non-trivial task that requires knowledge of all these curves for all available hardware components as well as all available fluorochromes.
Czech name
—
Czech description
—
Classification
Type
O - Miscellaneous
CEP classification
JD - Use of computers, robotics and its application
OECD FORD branch
—
Result continuities
Project
<a href="/en/project/LC535" target="_blank" >LC535: Chromosome dynamics and organization during the cell cycle in normal and pathologic conditions</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2006
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů