Bioinformatic prediction, molecular modelling and image analysis of localization and interactions of apoptotic proteins endonuclease G, AIF, and AMID

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F08%3A00024764" target="_blank" >RIV/00216224:14330/08:00024764 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Bioinformatic prediction, molecular modelling and image analysis of localization and interactions of apoptotic proteins endonuclease G, AIF, and AMID

Original language description

During apoptosis several mitochondrial proteins are released. They participate in caspase-independent nuclear DNA degradation, especially apoptosis-inducing factor (AIF, also PDCD8 or AIFM1) and endonuclease G. Another interesting protein, which was expected to act similarly as AIF due to the high sequence homology with AIF is AIF-homologous mitochondrion-associated inducer of death (AMID, also PRG3 or AIFM2). We studied the cellular localization and colocalization of proteins AIF, endonuclease G and AMID experimentally using vectors encoding proteins of interest fused to the fluorescent proteins and using bioinformatic predictions, that analyze the amino acid sequence of the proteins with various algorithms. We confirmed the colocalization of AIF andendonuclease G in the mitochondria of human cells. AMID was found to be the cytoplasmic protein, bound to various cellular surfaces from their cytoplasmic side. Overexpression of fusion protein AMID-HcRed-tandem was not lethal to the cell

Czech name

Bioinformatická předpověď, molekulární modelování a obrazová analýza polohy a interakcí apoptotických proteinů endonukleázy G, AIF a AMIDu

Czech description

Bioinformatická předpověď, molekulární modelování a obrazová analýza polohy a interakcí apoptotických proteinů endonukleázy G, AIF a AMIDu

Type

O - Miscellaneous

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů