Isolation of granulocytes: Which transcriptome do we analyze neutrophils or eosinophils?

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F10%3A00067250" target="_blank" >RIV/00216224:14330/10:00067250 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Isolation of granulocytes: Which transcriptome do we analyze neutrophils or eosinophils?

Original language description

Isolation of granulocyte cells from blood is necessary for accurate studying of changes in their expression. After gradient centrifugation, we obtain relatively pure granulocyte populations with different ratios of neutrophils and eosinophils. Unfortunately, in many studies in this field the expression results are not set according to the real variability of the granulocyte population. In many cases, the granulocyte population is marked simply as neutrophils and the residual population of eosinophils isnot considered. Based on our recent study where we tracked the general transcription factor RNA polymerase II (RNAP II), we hypothesized that eosinophils are more transcriptionally active cells than neutrophils. We decided to test our hypothesis on isolated cells because its implications could change our view on many past expression analyses performed on granulocytes. In our experiments, we isolated neutrophils and eosinophils and measured their total RNA production.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EC - Immunology

OECD FORD branch

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Project

<a href="/en/project/2B06052" target="_blank" >2B06052: Determination of markers, screening and early diagnostics of cancer diseases using highly automated processing of multidimensional biomedical images</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

FOLIA BIOLOGICA

ISSN

0015-5500

e-ISSN

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Volume of the periodical

56

Issue of the periodical within the volume

6

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

4

Pages from-to

252-255

UT code for WoS article

000286039500003

EID of the result in the Scopus database

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