Recruitment and activation of a lipid kinase by hepatitis C virus NS5A is essential for integrity of the membranous replication compartment.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F11%3A00052648" target="_blank" >RIV/00216224:14330/11:00052648 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.chom.2010.12.002" target="_blank" >http://dx.doi.org/10.1016/j.chom.2010.12.002</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chom.2010.12.002" target="_blank" >10.1016/j.chom.2010.12.002</a>

Result language

angličtina

Original language name

Recruitment and activation of a lipid kinase by hepatitis C virus NS5A is essential for integrity of the membranous replication compartment.

Original language description

Hepatitis C virus (HCV) is a major causative agent of chronic liver disease in humans. To gain insight into host factor requirements for HCV replication, we performed a siRNA screen of the human kinome and identified 13 different kinases, including phosphatidylinositol-4 kinase III alpha (PI4KIIIalfa), as being required for HCV replication. Consistent with elevated levels of the PI4KIIIalfa product phosphatidylinositol-4-phosphate (PI4P) detected in HCV-infected cultured hepatocytes and liver tissue from chronic hepatitis C patients, the enzymatic activity of PI4KIIIalfa was critical for HCV replication. Viral nonstructural protein 5A (NS5A) was found to interact with PI4KIIIalfa and stimulate its kinase activity. The absence of PI4KIIIalfa activity induced a dramatic change in the ultrastructural morphology of the membranous HCV replication complex.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

—

Project

<a href="/en/project/2B06052" target="_blank" >2B06052: Determination of markers, screening and early diagnostics of cancer diseases using highly automated processing of multidimensional biomedical images</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cell Host & Microbe

ISSN

1931-3128

e-ISSN

—

Volume of the periodical

9

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

14

Pages from-to

32-45

UT code for WoS article

000287344500006

EID of the result in the Scopus database

—