Endonuclease G interacts with histone H2B and DNA topoisomerase II alpha during apoptosis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F12%3A00059138" target="_blank" >RIV/00216224:14330/12:00059138 - isvavai.cz</a>

Result on the web

<a href="http://link.springer.com/article/10.1007%2Fs11010-011-1182-x" target="_blank" >http://link.springer.com/article/10.1007%2Fs11010-011-1182-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11010-011-1182-x" target="_blank" >10.1007/s11010-011-1182-x</a>

Result language

angličtina

Original language name

Endonuclease G interacts with histone H2B and DNA topoisomerase II alpha during apoptosis

Original language description

During some apoptotic pathways, proteins apoptosis-inducing factor (AIF) and endonuclease G (EndoG) are released from the mitochondria and they translocate into the cell nuclei, where they probably participate in chromatin degradation together with othernuclear proteins. Exact mechanism of EndoG activity in cell nucleus is still unknown. We conducted a living-cell confocal fluorescence microscopy followed by an image analysis of fluorescence resonance energy transfer (FRET) to analyze the possibility of protein interactions of EndoG with histone H2B and human DNA topoisomerase II alpha (TOPO2a). Our results show that EndoG interacts with both these proteins during apoptotic cell death. Therefore, we can conclude that EndoG and TOPO2a may actively participate in apoptotic chromatin degradation. The possible existence of a degradation complex consisting of EndoG and TOPO2a and possibly other proteins like AIF and cyclophilin A have yet to be investigated.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

—

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Mol Cell Biochem

ISSN

0300-8177

e-ISSN

—

Volume of the periodical

363

Issue of the periodical within the volume

1-2

Country of publishing house

DE - GERMANY

Number of pages

7

Pages from-to

301-307

UT code for WoS article

000300888900030

EID of the result in the Scopus database

—