Live cell assays to identify regulators of ER to Golgi trafficking
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F12%3A00059140" target="_blank" >RIV/00216224:14330/12:00059140 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1111/j.1600-0854.2011.01318.x" target="_blank" >http://dx.doi.org/10.1111/j.1600-0854.2011.01318.x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/j.1600-0854.2011.01318.x" target="_blank" >10.1111/j.1600-0854.2011.01318.x</a>
Alternative languages
Result language
angličtina
Original language name
Live cell assays to identify regulators of ER to Golgi trafficking
Original language description
We used fluorescence microscopy based quantitative assays in living cells to identify regulators of ER to Golgi trafficking and/or Golgi complex maintenance. We first validated an automated procedure to identify factors, which influence Golgi to ER re-localization of GalT-CFP after BFA addition and wash-out. We then tested 14 proteins that potentially play a role in ER to Golgi trafficking, and localize to the ER and/or Golgi complex when over-expressed. 9 of them interfered with the rate of BFA inducedredistribution of GalT-CFP to the ER, 6 of them interfered with GalT-CFP reassembly rate to a juxtanuclear region after BFA wash-out, and 6 of them were positive effectors in both assays. Notably, our live cell approach captures functions of those regulators of ER to Golgi trafficking, which were missed in previous fixed cell assays; as well as assigns respective roles for yet incompletely characterized proteins.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EA - Morphology and cytology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/2B06052" target="_blank" >2B06052: Determination of markers, screening and early diagnostics of cancer diseases using highly automated processing of multidimensional biomedical images</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Traffic
ISSN
1398-9219
e-ISSN
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Volume of the periodical
13
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
17
Pages from-to
416-432
UT code for WoS article
000300054100006
EID of the result in the Scopus database
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