DNA double strand breaks detection in pluripotent stem cells with regard to cell cycle stages

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F16%3A00088039" target="_blank" >RIV/00216224:14330/16:00088039 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
DNA double strand breaks detection in pluripotent stem cells with regard to cell cycle stages
Original language description
Human induced pluripotent stem cells (hiPSCs) have a potential in both disease modeling and regenerative medicine. It is of utmost importance that genomic integrity of the cells remains unharmed and DNA reparation systems fully functional. In our research we focus on the detection of DNA double strand breaks (DSBs) by phosphorylated histone H2AX (known as gammaH2AX) and p53-binding protein 1 (53BP1) in fibroblasts, three distinct lines of hiPSCs, and one line of human embryonic stem cells (hESCs). We measured both spontaneously occurred DSBs and DSBs induced by gamma-irradiation and its decrease in time. Foci number was detected by fluorescence microscopy and EdU (5-ethynyl-2-deoxyuridine) was used to discriminate between cell cycle stages. Discrimination between the EdU negative (G1) and positive (S/G2) populations allows excluding the replication-related foci and increase the accuracy of measurement. This is crucial when comparing number of DSBs in cell types with different cell cycle speed (ie.
Czech name
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Czech description
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Project
<a href="/en/project/GBP302%2F12%2FG157" target="_blank" >GBP302/12/G157: Dynamics and Organization of Chromosomes in the Cell Cycle and during Differentiation under Normal and Pathological Conditions</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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