Toward Robust Fully 3D Filopodium Segmentation and Tracking in Time-Lapse Fluorescence Microscopy
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F19%3A00107396" target="_blank" >RIV/00216224:14330/19:00107396 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1109/ICIP.2019.8803721" target="_blank" >http://dx.doi.org/10.1109/ICIP.2019.8803721</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1109/ICIP.2019.8803721" target="_blank" >10.1109/ICIP.2019.8803721</a>
Alternative languages
Result language
angličtina
Original language name
Toward Robust Fully 3D Filopodium Segmentation and Tracking in Time-Lapse Fluorescence Microscopy
Original language description
Development, parameter tuning, and objective benchmarking of bioimage analysis workflows heavily rely on the availability of diverse bioimage datasets accompanied by reference annotations. In this paper, we present a new benchmark dataset, FiloData3D, designed for in-depth performance assessments of fully 3D filopodium segmentation and tracking algorithms that emerged recently in the field. It consists of 180 synthetic, fully annotated, 3D time-lapse sequences of single lung cancer cells, combining different cell shapes, signal-to-noise ratios, and anisotropy ratios, which are the well-known factors that influence the quality of segmentation and tracking results. Using FiloData3D, we show that the number of filopodia and their lengths extracted are significantly underestimated in the case of traditional 2D protocols that prevail in daily practice compared to fully 3D measurements, calling for a procedural change in filopodial analyses of 3D+t bioimage data.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
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OECD FORD branch
10201 - Computer sciences, information science, bioinformathics (hardware development to be 2.2, social aspect to be 5.8)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
26th IEEE International Conference on Image Processing
ISBN
9781538662496
ISSN
1522-4880
e-ISSN
—
Number of pages
5
Pages from-to
819-823
Publisher name
IEEE
Place of publication
Taipei
Event location
Taipei, Taiwan
Event date
Jan 1, 2019
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000521828600163