THE EFFECT OF MATING FACTOR ON YARROWIA LIPOLYTICA
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14410%2F07%3A00032805" target="_blank" >RIV/00216224:14410/07:00032805 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
THE EFFECT OF MATING FACTOR ON YARROWIA LIPOLYTICA
Original language description
Yarrowia lipolytica has two mating types designated A and B. Mating type A cells produce mating specific pheromone (A factor). Crude fractions of the A factor was partially purified and concentrated (1.000 fold) by Amberlite CG 50. This A factor acts only on cells of opposite mating type inducning a cascade of metabolic and morphological changes. Ths A factor specially inhibits the cell division of mating type B. The cell were arrested in the G1 phase (no budding, characterized by light microscopy). Thearrest is reversible and the lenght of the arrest period is dependent on the concentration of A factor added. The A factor is one element of a relatory system whose functions assure the synchronization of haploid cells for conjugation. In an asynchronous population only a small part of cells are in this specific stage. When A factor is added to conjugation medium (YM), the time for conjugation was shorter and the conjugation rate was increased.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2007
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů