Analysis of Plant Meiotic Chromosomes by Chromosome Painting
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F13%3A00065831" target="_blank" >RIV/00216224:14740/13:00065831 - isvavai.cz</a>
Result on the web
<a href="http://www.springerprotocols.com/BookToc/doi/10.1007/978-1-62703-333-6" target="_blank" >http://www.springerprotocols.com/BookToc/doi/10.1007/978-1-62703-333-6</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-62703-333-6_2" target="_blank" >10.1007/978-1-62703-333-6_2</a>
Alternative languages
Result language
angličtina
Original language name
Analysis of Plant Meiotic Chromosomes by Chromosome Painting
Original language description
Chromosome painting (CP) refers to visualization of large chromosome regions, entire chromosome arms, or entire chromosomes via fluorescence in situ hybridization (FISH). For CP in plants, contigs of chromosome-specific bacterial artificial chromosomes (BAC) from the target species or from a closely related species (comparative chromosome painting, CCP) are typically applied as painting probes. Extended pachytene chromosomes provide the highest resolution of CP in plants. CP enables identification and tracing of particular chromosome regions and/or entire chromosomes throughout all meiotic stages as well as corresponding chromosome territories in premeiotic interphase nuclei. Meiotic pairing and structural chromosome rearrangements (typically inversions and translocations) can be identified by CP. Here, we describe step-by-step protocols of CP and CCP in plant species including chromosome preparation, BAC DNA labeling, and multicolor FISH.
Czech name
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Czech description
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Classification
Type
C - Chapter in a specialist book
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Book/collection name
Plant Meiosis: Methods and Protocols
ISBN
9781627033329
Number of pages of the result
12
Pages from-to
13-24
Number of pages of the book
238
Publisher name
Humana Press
Place of publication
New York
UT code for WoS chapter
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