Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of AHP2, a signal transmitter protein from Arabidopsis thaliana
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F13%3A00066056" target="_blank" >RIV/00216224:14740/13:00066056 - isvavai.cz</a>
Alternative codes found
RIV/67179843:_____/13:00399198 RIV/60076658:12520/13:43885181
Result on the web
<a href="http://journals.iucr.org/f/issues/2013/02/00/gj5115/stdsup.html" target="_blank" >http://journals.iucr.org/f/issues/2013/02/00/gj5115/stdsup.html</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1107/S174430911205186X" target="_blank" >10.1107/S174430911205186X</a>
Alternative languages
Result language
angličtina
Original language name
Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of AHP2, a signal transmitter protein from Arabidopsis thaliana
Original language description
Histidine-containing phosphotransfer proteins from Arabidopsis thaliana(AHP1?5) act as intermediates between sensor histidine kinases and response regulators in a signalling system called multi-step phosphorelay (MSP). AHP proteins mediate and potentially integrate various MSP-based signalling pathways (e.g. cytokinin or osmosensing). However, structural information about AHP proteins and their importance in MSP signalling is still lacking. To obtain a deeper insight into the structural basis of AHP-mediated signal transduction, the three-dimensional structure of AHP2 was determined. The AHP2 coding sequence was cloned into pRSET B expression vector, enabling production of AHP2 fused to an N-terminal His tag. AHP2 was expressed in soluble form in Escherichia coli strain BL21 (DE3) pLysS and then purified to homogeneity using metal chelate affinity chromatography and anion-exchange chromatography under reducing conditions.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Acta Crystalographica Section F
ISSN
1744-3091
e-ISSN
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Volume of the periodical
69
Issue of the periodical within the volume
2
Country of publishing house
US - UNITED STATES
Number of pages
4
Pages from-to
158-161
UT code for WoS article
000314467200017
EID of the result in the Scopus database
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