Crystallization and preliminary X-ray crystallographic analysis of recombinant beta-mannosidase from Aspergillus niger
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F13%3A00066401" target="_blank" >RIV/00216224:14740/13:00066401 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
čeština
Original language name
Crystallization and preliminary X-ray crystallographic analysis of recombinant beta-mannosidase from Aspergillus niger
Original language description
We report the crystallization and preliminary X-ray crystallographic analysis of recombinant beta-mannosidase overexpressed in Pichia pastoris. The best crystals were produced by further optimization using the hanging-drop vapour diffusion method. Diffraction data were collected at BESSY II Berlin (14.1 and 14.2). The data were processed by XDSAPP. The crystals belonged to space group P1. The beta-mannosidase in the native data set diffracted to 2.41 A resolution. Experimental phasing, model fitting andrefinement are in progress.
Czech name
Crystallization and preliminary X-ray crystallographic analysis of recombinant beta-mannosidase from Aspergillus niger
Czech description
We report the crystallization and preliminary X-ray crystallographic analysis of recombinant beta-mannosidase overexpressed in Pichia pastoris. The best crystals were produced by further optimization using the hanging-drop vapour diffusion method. Diffraction data were collected at BESSY II Berlin (14.1 and 14.2). The data were processed by XDSAPP. The crystals belonged to space group P1. The beta-mannosidase in the native data set diffracted to 2.41 A resolution. Experimental phasing, model fitting andrefinement are in progress.
Classification
Type
O - Miscellaneous
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GAP207%2F10%2F0321" target="_blank" >GAP207/10/0321: The ways to N-acetyl-mannosamine structures via mannoside metabolizing enzymes</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů