The influence of culture conditions on the identification of Mycobacterium species by MALDI-TOF MS profiling
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F14%3A00073252" target="_blank" >RIV/00216224:14740/14:00073252 - isvavai.cz</a>
Alternative codes found
RIV/00027162:_____/14:#0001094
Result on the web
<a href="http://onlinelibrary.wiley.com/doi/10.1111/1574-6968.12408/epdf" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/1574-6968.12408/epdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/1574-6968.12408" target="_blank" >10.1111/1574-6968.12408</a>
Alternative languages
Result language
angličtina
Original language name
The influence of culture conditions on the identification of Mycobacterium species by MALDI-TOF MS profiling
Original language description
Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) represents a simple reliable approach for rapid bacterial identification based on specific peptide/protein fingerprints. However, cell-wall characteristics of mycobacterial species, and their well known stability, complicate MALDI-TOF MS profiling analysis. In this study, we tested two recently published protocols for inactivation and disruption of mycobacteria, and we also examined the influence of different culture conditions (four culture media and five cultivation times) on mass spectral quality and the discriminatory power of the method. We found a significant influence of sample pretreatment method and culture medium on species identification and differentiation for a total of 10 strains belonging to Mycobacterium phlei and Mycobacteriumsmegmatis.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
FEMS Microbiology Letters
ISSN
0378-1097
e-ISSN
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Volume of the periodical
353
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
77-84
UT code for WoS article
000333654500011
EID of the result in the Scopus database
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