Loss of loop adenines alters human telomere d(AG3(TTAG3)3) quadruplex folding
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F14%3A00073361" target="_blank" >RIV/00216224:14740/14:00073361 - isvavai.cz</a>
Alternative codes found
RIV/68081707:_____/14:00440266
Result on the web
<a href="http://dx.doi.org/10.1093/nar/gku1245" target="_blank" >http://dx.doi.org/10.1093/nar/gku1245</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/nar/gku1245" target="_blank" >10.1093/nar/gku1245</a>
Alternative languages
Result language
angličtina
Original language name
Loss of loop adenines alters human telomere d(AG3(TTAG3)3) quadruplex folding
Original language description
Abasic (AP) lesions are the most frequent type of damages occurring in cellular DNA. Here we describe the conformational effects of AP sites substituted for 2'-deoxyadenosine in the first (ap7), second (ap13) or third (ap19) loop of the quadruplex formedin K+ by the human telomere DNA 5'-d[AG3(TTAG3)3]. CD spectra and electrophoresis reveal that the presence of AP sites does not hinder the formation of intramolecular quadruplexes. NMR spectra show that the structural heterogeneity is substantially reduced in ap7 and ap19 as compared to that in the wild-type. These two (ap7 and ap19) sequences are shown to adopt the hybrid-1 and hybrid-2 quadruplex topology, respectively, with AP site located in a propeller-like loop. All three studied sequences transform easily into parallel quadruplex in dehydrating ethanol solution. Thus, the AP site in any loop region facilitates the formation of the propeller loop.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nucleic Acids Research
ISSN
0305-1048
e-ISSN
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Volume of the periodical
42
Issue of the periodical within the volume
22
Country of publishing house
GB - UNITED KINGDOM
Number of pages
11
Pages from-to
14031-14041
UT code for WoS article
000347916900057
EID of the result in the Scopus database
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