Spectral density mapping protocols for analysis of molecular motions in disordered proteins

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F14%3A00073529" target="_blank" >RIV/00216224:14740/14:00073529 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/14:00439577

Result on the web

<a href="http://link.springer.com/article/10.1007%2Fs10858-014-9816-4" target="_blank" >http://link.springer.com/article/10.1007%2Fs10858-014-9816-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10858-014-9816-4" target="_blank" >10.1007/s10858-014-9816-4</a>

Result language

angličtina

Original language name

Spectral density mapping protocols for analysis of molecular motions in disordered proteins

Original language description

Spectral density mapping represents the method of choice for investigations of molecular motions of intrinsically disordered proteins (IDPs). However, the current methodology has been developed for well-folded proteins. In order to find conditions for areliable analysis of relaxation of IDPs, accuracy of the current reduced spectral density mapping protocols applied to IDPs was examined and new spectral density mapping methods employing cross-correlated relaxation rates have been designed. Various sources of possible systematic errors were analyzed theoretically and the presented approaches were tested on a partially disordered protein, delta subunit of bacterial RNA polymerase. Results showed that the proposed protocols provide unbiased description of molecular motions of IDPs and allow to separate slow exchange from fast dynamics.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Biomolecular NMR

ISSN

0925-2738

e-ISSN

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Volume of the periodical

58

Issue of the periodical within the volume

3

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

15

Pages from-to

193-207

UT code for WoS article

000332743800005

EID of the result in the Scopus database

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