X-ray vs. NMR structure of N-terminal domain of delta-subunit of RNA polymerase
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F14%3A00073722" target="_blank" >RIV/00216224:14740/14:00073722 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/14:00433309
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S1047847714001373" target="_blank" >http://www.sciencedirect.com/science/article/pii/S1047847714001373</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jsb.2014.06.001" target="_blank" >10.1016/j.jsb.2014.06.001</a>
Alternative languages
Result language
angličtina
Original language name
X-ray vs. NMR structure of N-terminal domain of delta-subunit of RNA polymerase
Original language description
The crystal structure of the N-terminal domain of the RNA polymerase delta subunit (Ndelta) from Bacillus subtilis solved at a resolution of 2.0 A is compared with the NMR structure determined previously. The molecule crystallizes in the space group C222(1) with a dimer in the asymmetric unit. Importantly, the X-ray structure exhibits significant differences from the lowest energy NMR structure. In addition to the overall structure differences, structurally important beta sheets found in the NMR structure are not present in the crystal structure. We systematically investigated the cause of the discrepancies between the NMR and X-ray structures of Ndelta, addressing the pH dependence, presence of metal ions, and crystal packing forces. We convincingly showed that the crystal packing forces, together with the presence of Ni2+ ions, are the main reason for such a difference.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Structural Biology
ISSN
1047-8477
e-ISSN
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Volume of the periodical
187
Issue of the periodical within the volume
2
Country of publishing house
US - UNITED STATES
Number of pages
13
Pages from-to
174-186
UT code for WoS article
000340217300008
EID of the result in the Scopus database
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