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New sensitive detection method for lectin hemagglutination using microscopy.

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F14%3A00073809" target="_blank" >RIV/00216224:14740/14:00073809 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1002/jemt.22407" target="_blank" >http://dx.doi.org/10.1002/jemt.22407</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1002/jemt.22407" target="_blank" >10.1002/jemt.22407</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    New sensitive detection method for lectin hemagglutination using microscopy.

  • Original language description

    The blood group system AB0 is determined by the composition of terminal oligosaccharides on red blood cells. Thanks to this structural feature, these groups can be recognized by saccharide-recognizing compounds. Hemagglutination is a process in which blood cells are cross-linked via multivalent molecules, lectins e.g. A hemagglutination assay is commonly used for the detection of multivalent molecules that recognize blood cells, in order to search for their sugar specificity. It is traditionally performed on a microtiter plate, where the lectin solution is serially diluted and the lowest concentration of lectin causing agglutination is detected. We have developed a new way of detecting hemagglutination using microscopy, which was tested on purified lectins as well as cell lysates. Hemagglutination was performed on a microscope slide directly and detected using a microscope.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CE - Biochemistry

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2014

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Microscopy research and technique

  • ISSN

    1059-910X

  • e-ISSN

  • Volume of the periodical

    77

  • Issue of the periodical within the volume

    10

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    9

  • Pages from-to

    841-849

  • UT code for WoS article

    000342207900012

  • EID of the result in the Scopus database