Detection of Aerosolized Biological Agents Using the Piezoelectric Immunosensor
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F14%3A00076277" target="_blank" >RIV/00216224:14740/14:00076277 - isvavai.cz</a>
Result on the web
<a href="http://pubs.acs.org/doi/abs/10.1021/ac501623m" target="_blank" >http://pubs.acs.org/doi/abs/10.1021/ac501623m</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/ac501623m" target="_blank" >10.1021/ac501623m</a>
Alternative languages
Result language
angličtina
Original language name
Detection of Aerosolized Biological Agents Using the Piezoelectric Immunosensor
Original language description
Airborne microorganisms are a major cause of respiratory diseases. Detection of pathogenic bacteria in the form of bioaerosols is required not only in peacetime but also in the threat of biological attacks. The label-free and direct detection of aerosolized biological agents is presented here. A desktop bioaerosol chamber for safe work with aerosolized microbial cells was constructed, and its functionality was tested. The model organisms (Escherichia coli) were disseminated using an aerosol generator inthe chamber filled with either common laboratory indoor air or sterile air. The particles from the generated aerosol were collected using the cyclone SASS 2300, suspended in buffer and then analyzed using the piezoelectric immunosensor modified with specific capture antibodies. The frequency shifts indicated presence of the model biological agent with limit of detection of 1.45 x 104 CFU x L?1 of air. The total time from sample collection to detection was 16 min.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: Central european institute of technology</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytical Chemistry
ISSN
0003-2700
e-ISSN
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Volume of the periodical
86
Issue of the periodical within the volume
17
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
8680-8686
UT code for WoS article
000341229200026
EID of the result in the Scopus database
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