A Novel Protein-Protein Interaction in the RES (REtention and Splicing) Complex
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F14%3A00079761" target="_blank" >RIV/00216224:14740/14:00079761 - isvavai.cz</a>
Result on the web
<a href="http://www.jbc.org/content/289/41/28640.long" target="_blank" >http://www.jbc.org/content/289/41/28640.long</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1074/jbc.M114.592311" target="_blank" >10.1074/jbc.M114.592311</a>
Alternative languages
Result language
angličtina
Original language name
A Novel Protein-Protein Interaction in the RES (REtention and Splicing) Complex
Original language description
The retention and splicing (RES) complex is a conserved spliceosome-associated module that was shown to enhance splicing of a subset of transcripts and promote the nuclear retention of unspliced pre-mRNAs in yeast. The heterotrimeric RES complex is organized around the Snu17p protein that binds to both the Bud13p and Pml1p subunits. Snu17p exhibits an RRM domain that resembles a U2AF homology motif (UHM) and Bud13p harbors a Trp residue reminiscent of an UHM-ligand motif (ULM). It has therefore been proposed that the interaction between Snu17p and Bud13p resembles canonical UHM-ULM complexes. Here, we have used biochemical and NMR structural analysis to characterize the structure of the yeast Snu17p-Bud13p complex. Unlike known UHMs that sequester theTrp residue of the ULM ligand in a hydrophobic pocket, Snu17p and Bud13p utilize a large interaction surface formed around the two helices of the Snu17p domain.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/EE2.3.20.0042" target="_blank" >EE2.3.20.0042: Internationalisation of the Structural Biology Research Programme with Emphasis on New Directions in R&D</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Biological Chemistry
ISSN
0021-9258
e-ISSN
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Volume of the periodical
289
Issue of the periodical within the volume
41
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
28640-28650
UT code for WoS article
000343765400052
EID of the result in the Scopus database
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