Single-breath analysis using a novel simple sampler and capillary electrophoresis with contactless conductometric detection

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F15%3A00081636" target="_blank" >RIV/00216224:14740/15:00081636 - isvavai.cz</a>

Result on the web

<a href="http://onlinelibrary.wiley.com/doi/10.1002/elps.201400456/abstract" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/elps.201400456/abstract</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elps.201400456" target="_blank" >10.1002/elps.201400456</a>

Result language

angličtina

Original language name

Single-breath analysis using a novel simple sampler and capillary electrophoresis with contactless conductometric detection

Original language description

The analysis of ionic content of exhaled breath condensate (EBC) from one single breath by CE with (CD)-D-4 is demonstrated for the first time. A miniature sampler made from a 2-mL syringe and an aluminum cooling cylinder for collection of EBC was developed. Various parameters of the sampler that influence its collection efficiency, repeatability, and effect of respiratory patterns were studied in detail. Efficient procedures for the cleanup of the miniature sampler were also developed and resulted in significant improvement of sampling repeatability. Analysis of EBC was performed by CE-(CD)-D-4 in a 60 mM MES/L-histidine BGE with 30 mu M CTAB and 2 mM 18-crown-6 at pH 6 and excellent repeatability of migration times (RSD &lt; 1.3% (n = 7)) and peak areas (RSD &lt; 7% (n = 7)) of 12 inorganic anions, cations, and organic acids was obtained. It has been shown that the breathing pattern has a significant impact on the concentration of the analytes in the collected EBC.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electrophoresis

ISSN

0173-0835

e-ISSN

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Volume of the periodical

36

Issue of the periodical within the volume

4

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

526-533

UT code for WoS article

000349969700006

EID of the result in the Scopus database

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