Sample preparation for N-glycosylation analysis of therapeutic monoclonal antibodies by electrophoresis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F15%3A00082381" target="_blank" >RIV/00216224:14740/15:00082381 - isvavai.cz</a>

Alternative codes found

RIV/68081715:_____/15:00444012

Result on the web

<a href="https://link.springer.com/protocol/10.1007%2F978-1-4939-2353-3_16" target="_blank" >https://link.springer.com/protocol/10.1007%2F978-1-4939-2353-3_16</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-4939-2353-3_16" target="_blank" >10.1007/978-1-4939-2353-3_16</a>

Result language

angličtina

Original language name

Sample preparation for N-glycosylation analysis of therapeutic monoclonal antibodies by electrophoresis

Original language description

There are a considerable number of biopharmaceuticals that have been approved for clinical use in the past decade. Over half of these new generation drugs are glycoproteins, such as monoclonal antibodies or other recombinant glycoproteins, which are mostly produced in mammalian cell lines. The linked carbohydrate moieties affect not only their physicochemical properties and thermal stability but also crucial features like receptor-binding activity, circulating half-life, as well as immunogenicity. The structural diversity of these attached glycans can be manifested in altered monosaccharide composition and linkages/positions among the monosaccharide building blocks. In addition, as more and more biosimilar products hit the market, understanding the effects of their glycosylation modification has become a recent target in efficacy and safety issues. To ensure consistent quality of these products, glycosylation profiles have to be monitored and controlled in all steps of the manufacturin

Czech name

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Czech description

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Type

C - Chapter in a specialist book

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/GAP301%2F11%2F2055" target="_blank" >GAP301/11/2055: New methods of analysis of proteins and their glycosylation in cancer - combination of electrochemistry, microfludic biosensors and mass spectrometry.</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Book/collection name

Methods in Molecular Biology

ISBN

9781493923526

Number of pages of the result

13

Pages from-to

183-195

Number of pages of the book

200

Publisher name

Springer Science+Business Media

Place of publication

NEW YORK

UT code for WoS chapter

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