Assessment of p53 and ATM functionality in chronic lymphocytic leukemia by multiplex ligation-dependent probe amplification

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F15%3A00085031" target="_blank" >RIV/00216224:14740/15:00085031 - isvavai.cz</a>

Result on the web

<a href="http://www.nature.com/cddis/journal/v6/n8/pdf/cddis2015223a.pdf" target="_blank" >http://www.nature.com/cddis/journal/v6/n8/pdf/cddis2015223a.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/cddis.2015.223" target="_blank" >10.1038/cddis.2015.223</a>

Result language

angličtina

Original language name

Assessment of p53 and ATM functionality in chronic lymphocytic leukemia by multiplex ligation-dependent probe amplification

Original language description

The ATM-p53 DNA-damage response (DDR) pathway has a crucial role in chemoresistance in CLL, as indicated by the adverse prognostic impact of genetic aberrations of TP53 and ATM. Identifying and distinguishing TP53 and ATM functional defects has become relevant as epigenetic and posttranscriptional dysregulation of the ATM/p53 axis is increasingly being recognized as the underlying cause of chemoresistance. Also, specific treatments sensitizing TP53-or ATM-deficient CLL cells are emerging. We therefore developed a new ATM-p53 functional assay with the aim to (i) identify and (ii) distinguish abnormalities of TP53 versus ATM and (iii) enable the identification of additional defects in the ATM-p53 pathway. Reversed transcriptase multiplex ligation-dependent probe amplification (RT-MLPA) was used to measure ATM and/or p53-dependent genes at the RNA level following DNA damage using irradiation. Here, we showed that this assay is able to identify and distinguish three subgroups of CLL tumors

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

CELL DEATH & DISEASE

ISSN

2041-4889

e-ISSN

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Volume of the periodical

6

Issue of the periodical within the volume

august

Country of publishing house

GB - UNITED KINGDOM

Number of pages

8

Pages from-to

"nestránkováno"

UT code for WoS article

000360581900016

EID of the result in the Scopus database

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