Comparative study reveals better far-red fluorescent protein for whole body imaging

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F15%3A00085159" target="_blank" >RIV/00216224:14740/15:00085159 - isvavai.cz</a>

Result on the web

<a href="http://www.nature.com/articles/srep10332" target="_blank" >http://www.nature.com/articles/srep10332</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/srep10332" target="_blank" >10.1038/srep10332</a>

Result language

angličtina

Original language name

Comparative study reveals better far-red fluorescent protein for whole body imaging

Original language description

Genetically encoded far-red and near-infrared fluorescent proteins enable efficient imaging in studies of tumorigenesis, embryogenesis, and inflammation in model animals. Here we report comparative testing of available GFP-like far-red fluorescent proteins along with a modified protein, named Katushka2S, and near-infrared bacterial phytochrome-based markers. We compare fluorescence signal and signal-to-noise ratio at various excitation wavelength and emission filter combinations using transiently transfected cell implants in mice, providing a basis for rational choice of optimal marker(s) for in vivo imaging studies. We demonstrate that the signals of various far-red fluorescent proteins can be spectrally unmixed based on different signal-to-noise ratios in different channels, providing the straightforward possibility of multiplexed imaging with standard equipment. Katushka2S produced the brightest and fastest maturing fluorescence in all experimental setups.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Scientific Reports

ISSN

2045-2322

e-ISSN

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Volume of the periodical

5

Issue of the periodical within the volume

June

Country of publishing house

GB - UNITED KINGDOM

Number of pages

9

Pages from-to

"nestránkováno"

UT code for WoS article

000355608400001

EID of the result in the Scopus database

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